The E.Z.N.A.® Mollusc DNA kit is designed for efficient purification of genomic DNA from molluscs, arthropods, round worms, flatworms and other invertebrate tissues rich in mucopolysaccharides. Fresh and frozen samples that have been preserved in alcohol or DNE can be used with this kit. This procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind matrix. Samples are homogenised and chloroform is added to remove mucopolysaccharides. Following a rapid alcohol precipitation step, DNA is further purified and all salts, proteins and other contaminants are removed. High quality genomic DNA is suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridisation techniques.
- DNA isolation under 20 minutes following lysis
- Optimised buffer system guarantee pure DNA
- Purified DNA suitable for any application
- Isolate DNA from a wide range of samples