TriFast™ is a complete ready to use reagent for simultaneous isolation of RNA, DNA and proteins from liquid samples, tissue and cells.
- Contains phenol and guanidine isothiocyanate
- Scalable prep size
- Suitable for small and large samples of human, animal, plant, yeast and virus
The isolation method for RNA, DNA and proteins is built on the well-known single step liquid phase separation (RNAClean). After addition of chloroform the homogenate separates into three phases upon centrifugation. The aqueous phase containing the RNA, the interphase the DNA and the organic phase the protein are processed separately resulting in RNA, DNA and protein of excellent quality in high yield.
Simultaneous purification of DNA is an effective way to normalize RNA yields from sample to sample.
Caution: Store at 4 °C, protect from long exposures to light (Stable for 12 months)