Total dietary fiber assay kit, Supelco®

Supplier: Merck
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TDF100A-1KTEA 368.4 GBP
TDF100A-1KT
Total dietary fiber assay kit, Supelco®
Food and Beverage Test Systems
This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinised with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample.

Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analysed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.
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