One-Wash™ Lentivirus titer kit, p24 ELISA

Supplier: OriGene
Ratings: (No Reviews)

Total Ratings: 0
Avg. Ratings: 0.0 out of 5

One-Wash™
ORIGTR30038EA 888 GBP
ORIGTR30038
One-Wash™ Lentivirus titer kit, p24 ELISA
Assays ELISAs
One-Wash™ Lentivirus Titer Kit, p24 ELISA for quantitative detection of HIV-1 p24 antigen in cell culture supernates for lentiviral particles titration.

  • Simplified workflow: One wash instead of four in the conventional ELISA protocol
  • One temperature incubation: No need for 37 °C incubation
  • Shortened sample binding time: 2 hour vs overnight

Lentivirus is a powerful gene delivery tool. To ensure efficient gene expression/knockdown, the optimal MOI (Multiplicity Of Infection) for specific cells needs to be tested; therefore lentiviral titer needs to be measured.

Many lentiviral vectors are derived from HIV-1. HIV-1 p24 is the viral capsid protein. p24 ELISA assays are developed to measure the amount of p24 in the lentivirus samples which is proportional to the amount of lentiviral particles. p24 ELISA is a standard sandwich enzyme-linked immune-sorbent assay.

A mouse monoclonal antibody specific for HIV-1 p24 is pre-coated onto 96-well plates. The p24 antigen in the specimen is specifically captured onto the immobilised antibody during specimen incubation. The captured antigen is then reacted with a biotinylated rabbit polyclonal HIV-1 p24 detection antibody.

Delivery information: Kit contains HIV-1 p24 antibody coated 96-well plate, recombinant HIV-1 p24 standard (100 µl), biotinylated HIV-1 p24 detection antibody (10 ml), HRP conjugated streptavidin-peroxidase (100 µl), sample lysis buffer (5 ml), assay diluent (25 ml), 10× plate wash buffer (25 ml), TMB substrate (10 ml), TMB stop solution (10 ml) and 2 plate sealer.

Caution: For research purposes only. Not intended for diagnostic use.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR