VWR ExoCleanUp FAST PCR reagent is a one-step PCR clean-up reagent for optimal sequencing results, consisting of a balanced combination of a heat-labile exonuclease I (HL-ExoI) and a recombinant shrimp alkaline phosphatase (rSAP).
- Designed to clean-up PCR products in 5 minutes
- No need for spin columns or magnetic beads
- Treatment improves downstream applications, such as DNA sequencing and SNP analysis
Treatment of amplified PCR products with this reagent helps to remove residual primers and single-stranded DNA, and inactivates excess dNTPs by dephosphorylation. After enzymatic treatment at 37 °C for a minimum of 2 minutes, this reagent is completely inactivated by heating at 80 °C for a minimum of 3 minutes.