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Anti-Cytokeratin 7 & 8 Mouse Monoclonal Antibody [clone: CAM 5.2]

Supplier: IMMUNOLOGIC
The Cytokeratin 7 & 8 antibody has a primary reactivity with human cytokeratin proteins that correspond to Moll's peptide 7 (cytokeratin 7) and Moll's peptide 8 (cytokeratin 8).1-4 These antigens are present on secretory epithelia of normal human tissue, but not on stratified squamous epithelium.1 Its antibody stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas. It may not react with some squamous cell carcinomas. 2, 5, 6

  • Control: Adenocarcinomas, pancreas, prostate, salivary gland
  • Dilution Range: 1:400-1:800 for immunohistochemical staining

References:
1. Moll R, et al, Cell. 1982;31:11-24
2. Makin C,et al, J Clin Pathol. 1984;37:975
3. Cooper D, et al, Lab In vest. 1985;52:243-256
4. Smedts F, et al, Am J Pathol. 1990;136:657-667
5. Battifora H.Progress in Surgical Pathology. Vol 8: Field & Wood Medical Publishers; 1990:1-15
6. Johnson A, et al, Br J Cancer. 1985;52:159-165
7. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3
8. Centers for Disease Control. MMWR. 1988;37:377-388

Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH 6,0 for 20 minutes or Protease K treatment for 10 min at 37 °C is required for IHC staining on formalin-fixed, paraffin embedded tissue sections.

Type:
Antigen: Cytokeratin 7 & 8
Clonality: Monoclonal
Clone: CAM 5.2
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human


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Size Pk VWR Catalog Number Quantity
1 mL 1 ml VWRKILM84911-C1
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SPECIFICATIONS

Antigen name Cytokeratin 7 & 8
Reactivity Human
Clonality Monoclonal
Clone CAM 5.2
Host Mouse
Isotype IgG2a
ImmunoChemistry Yes
Molecular Weight 53 kDa
Storage temperature +2 °C to +8 °C
Tested Applications Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

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