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Anti-KRT7/KRT8 Mouse Monoclonal Antibody [clone: CAM 5.2]

Supplier: IMMUNOLOGIC
The Cytokeratin 7 & 8 antibody has a primary reactivity with human cytokeratin proteins that correspond to Moll's peptide 7 (cytokeratin 7) and Moll's peptide 8 (cytokeratin 8).1-4 These antigens are present on secretory epithelia of normal human tissue, but not on stratified squamous epithelium.1 Its antibody stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas. It may not react with some squamous cell carcinomas. 2, 5, 6

  • Control: Adenocarcinomas, pancreas, prostate, salivary gland
  • Dilution Range: 1:400-1:800 for immunohistochemical staining

References:
1. Moll R, et al, Cell. 1982;31:11-24
2. Makin C,et al, J Clin Pathol. 1984;37:975
3. Cooper D, et al, Lab In vest. 1985;52:243-256
4. Smedts F, et al, Am J Pathol. 1990;136:657-667
5. Battifora H.Progress in Surgical Pathology. Vol 8: Field & Wood Medical Publishers; 1990:1-15
6. Johnson A, et al, Br J Cancer. 1985;52:159-165
7. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3
8. Centers for Disease Control. MMWR. 1988;37:377-388

Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH 6,0 for 20 minutes or Protease K treatment for 10 min at 37 °C is required for IHC staining on formalin-fixed, paraffin embedded tissue sections.

Type: Primary
Antigen: KRT7/KRT8
Clonality: Monoclonal
Clone: CAM 5.2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human



 

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Size Pk VWR Catalog Number Quantity
1 ml 1 ml VWRKILM84911-C1
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SPECIFICATIONS

Antigen symbol KRT7/KRT8
Antigen name Keratin 7/Keratin 8
Reactivity Human
Conjugation Unconjugated
Clonality Monoclonal
Antibody type Primary
Clone CAM 5.2
Host Mouse
Isotype IgG2a
ImmunoChemistry Yes
Antigen synonyms CYK8, CARD2, K7, CK8, SCL, K2C7, CK7, CYKER K2C8, KO, K8
Gene ID 3855, 3856
Molecular weight 53 kDa
Form Concentrated
Storage temperature 2°C - 8°C
Brand name Immunologic
Tested applications Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

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