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LDS sample loading buffer, non-reducing (4X)

Supplier: VWR Chemicals
LDS Sample Loading Buffer, Non-reducing (4X) is used to prepare protein samples for denaturing polyacrylamide gel electrophoresis using Bis-Tris and Laemmli gels. Mixing LDS Sample Loading Buffer with protein samples only denatures the proteins without reducing them.

  • Premixed for convenience
  • Requires cold storage
  • 4X concentration allows more volume for protein per lane
  • Bring to room temperature prior to use

Reducing agents such as DTT or 2-mercaptoethanol can be added to the buffer for protein sample preparation under reducing conditions. Glycerol is included in the loading buffer as a density agent to ensure the samples sink into the sample wells, while Coomassie serves as a tracking dye to monitor the progression of electrophoresis.


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Description Pk VWR Catalog Number Quantity
LDS sample loading buffer, non-reducing (4X), 5 ml 1 1B1669-5ML
LDS sample loading buffer, non-reducing (4X), 25 ml 1 1B1669-25ML
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