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Rapid equilbrium dialysis (RED) device, Pierce™

Supplier: Thermo Scientific
Single-Use RED (rapid equilibrium dialysis) Plate is composed of disposable high-density polypropylene and is pre-loaded with 48 equilibrium dialysis membrane inserts. Each insert is comprised of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane with varying molecular-weight cutoffs (8 or 12 kD MWCO).

  • Easy and ready to use
  • Design enables equilibrium to reach 200 min-1 agitation with in three to four hours
  • Reproducible and accurate for plasma binding assays
  • Robust compartmentalised design eliminates potential for leakage
  • For guaranteed performance each lot of inserts is functionally tested in a protein-binding assay

RED device is specifically designed and extensively validated for plasma serum binding assays. In addition to plasma protein binding, the device is used for determining drug partition between red blood cell and plasma; protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance; and the competition between tissue protein binding against plasma proteins. The single-use RED plate is validated for minimal nonspecific binding.

This format requires no extensive assembly steps or specialised equipment, and each chamber/well is easily accessible from the top of the device. The base plate has a standard 96-well plate footprint with 9×9 mm well spacing. Additionally, the high membrane surface-to-volume ratio allows rapid dialysis, where equilibrium can be reached in 4 hours with high levels of reproducibility and accuracy.



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Description MWCO Pk VWR Catalog Number Quantity
Single-use RED plate with inserts, 1 each 8 kD 1 PIER90006
Single-use RED plate with inserts, 5 each 8 kD 1 SET PIER90007
Single-use RED plate with inserts, 10 each 8 kD 10 PIER99006
Single-use RED plate with inserts, 1 each 12 kD 1 PIER90112
Single-use RED plate with inserts, 10 each 12 kD 10 PIER91012
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ACCESSORIES

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Accessories
Description Pk VWR Catalog Number Quantity
RED device inserts 50 PIER89809
RED device inserts 250 PIER89810
Single-use RED base plate (empty) 2 PIER90004
Single-use RED base plate (empty) 10 PIER90005
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