Keep my session open?
Ending In 
The session is expired
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

Thermal cyclers

Supplier: VWR Collection
The VWR® thermal cycler family combines high quality engineering with a comprehensive range of block formats. The UNO cycler is designed around a powerful yet easy to use software interface and uses the same platform for both 96-well and 384-well formats. The Doppio, with two independent high speed 48-well blocks in one system, offers the optimal solution for maximum flexibility within minimal footprint. The Ristretto is a compact personal cycler with the highest flexibility, having a universal block that can be loaded with up to either 32× 0,2 ml tubes or 16× 0,5 ml tubes with flat caps. Because of the special design of the heated-lid, the height adjusts automatically to the different tube sizes.

  • Outstanding performance: Thermal plate with impressive thermal characteristics; powerful Peltier elements with Long Life Technology; heat sink with cooling fans with magnetic bearing enabling rapid temperature change control of up to 5 °C/s (3 °C/s for Ristretto)
  • Reliable reproducible results: 8, 2×8 or16 Peltier elements control the temperature row by row, enabling an outstanding block homogeneity of ± 0,2 °C and the choice of absolute linear gradients and independent lane control
  • Simple to use: Sharp, clear TFT display and intuitive 'press screen' commands
  • Data control: Up to 4× USB (1× USB Ristretto), 1× Ethernet (MS Windows®, Linux), remote control and monitoring of instruments via PC software, MP3 signal tones, user calls via e-mail and master/slave control

All systems include simple to use PC software for remote control and monitoring of instruments, plus creating PCR protocols on the PC.

FlexGradient technology (option for UNO and Doppio only): With the temperature of the 8 rows each individually controlled, select between a perfectly linear temperature gradient (ideal for PCR optimisation), or independent lane control (ideal for the use of different primer pairs in the same run)
System tools at the touch of a button: Graphical or tabular programming, 'Global Program Ramp', 'Gradient Control', 'Tube Control', Emulation mode, Online help, 'Quickstart' function and 'Power Fail Denaturation' for auto-restart after power failure
High specification remote control: State of the art technology, with VWR ‘PCR Cycler Master Software’; receive notifications via email or use the system MP3 player
Emulation mode (UNO and Doppio only): For easy transfer of PCR protocols

Engineered and manufactured in Germany according to ISO 9001, calibrated and maintained according to NIST standards and backed with more than 20 years of cycler expertise and experience.



Expand All / Collapse All
Description Pk VWR Catalog Number Quantity
Thermal cycler, UNO96, with 96-well universal block and standard lid for 96× 0,2 ml tubes, 96-well PCR plates or 48× 0,5 ml tubes with flat caps 1 732-2548
Thermal cycler, UNO96 HPL, with 96-well universal block and high pressure lid (HPL, 100–250 N) for 96× 0,2 ml tubes, 96-well PCR plates or 48× 0,5 ml tubes with flat caps 1 732-2914
Gradient thermal cycler, UNO96 HPL Gradient, with 96-well universal gradient block and high pressure lid (HPL, 100–250 N) for 96× 0,2 ml tubes, 96-well PCR plates or 48× 0,5 ml tubes with flat caps 1 732-2915P
Gradient thermal cycler, UNO96 Gradient, with 96-well universal gradient block and standard lid for 96× 0,2 ml tubes, 96-well PCR plates or 48× 0,5 ml tubes with flat caps 1 732-2549P
Thermal cycler, UNO384, with 384-well block and high pressure lid for 384-well PCR plates 1 732-2550
Thermal cycler, Doppio, with 2× 48-well universal blocks and standard lids for 48× 0,2 ml tubes or 24× 0,5 ml tubes with flat caps per block 1 732-2551
Gradient thermal cycler, Doppio Gradient, with 2× 48-well universal gradient blocks and standard lids for 48× 0,2 ml tubes or 24× 0,5 ml tubes with flat caps per block 1 732-2552
Thermal cycler, Ristretto, with 32-well universal block and standard lid, for 32× 0,2 ml tubes, 4× 0,2 ml 8-strips, or 16× 0,5 ml tubes with flat caps 1 732-2553

Software for gradient upgrade
Description Pk VWR Catalog Number Quantity
Gradient upgrade for Doppio thermocycler 1 732-2555
Gradient upgrade for UNO96 thermocycler 1 732-2554
Generate Barcodes Barcode Label Format: 
Generate PDF Catalog Page


Model UNO96 HPL Doppio Ristretto Doppio Gradient UNO96 HPL Gradient UNO96 UNO384 UNO96 Gradient
Block accuracy (°C) ±0,1
Block homogeneity (°C) ±0,2 at 72 °C
Programs Unlimited number of programs via network PC or USB memory sticks: internal memory for 500000 typical PCR protocols
Temperature range (°C) +4...105
Lid temperature range (°C) +40...120 °C
Display Touch sensitive TFT display (800×480 px, 16:9, 65536 colours)
Gradient temperature range (°C) - +35...105 - +35...105
Heating and cooling rate (°C/sec) Max. 5 Max. 5 Max. 5
Interfaces 4× USB, 1× Ethernet (MS Windows®, Linux) 1× USB, 1× Ethernet (MS Windows®, Linux) 4× USB, 1× Ethernet (MS Windows®, Linux)


Need help?

  • Whatever you have to say, positive or negative, is important to us.
  • Providing feedback takes only seconds.
  • Your feedback will be made available to the public on the web. will publish your name as user.
You can use this name, or edit and save a nickname.
We recommend authors use their first and last initial.

Please confirm the industry you work in

Example: Pharmaceutical

Please confirm your role

Example: Clinical Trial Assistant

Please confirm your location

Example: New York, NY


Total Ratings: 0
Avg. Ratings: 0.0 out of 5

Sort all by:

Enter Details

Select the article for which you are looking for Certificate

Indicate the Lot Number. This field is mandatory. Enter 'x' if unknown for VWR Chemicals

Enter Certificate Details

Enter the Lot number:

Choose from recent batch/lot numbers:

Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty

Terms & Conditions

These terms and conditions cover all sales of products and services by VWR International Ltd (VWR) in the United Kingdom and any information and advice given whether charged for or not. No variation in these terms and conditions will apply unless agreed by VWR in writing. Orders are accepted only on the condition that no conditions of purchase shall apply. 


VWR has the right to change the prices shown in catalogues and price lists at any time and to invoice at prices current at the date of receipt of an order.

‘Price on application’ (POA) quotations and all other quotations will be valid for 30 days from the date of the quotation, unless otherwise notified by VWR.

All prices in catalogues and quotations are net of Value Added Tax and any other taxes or duties. These will be charged separately, if applicable. VWR reserve the right to apply a freight charge on all deliveries. 


Unless otherwise agreed in writing payment in full is due within 30 days from date of invoice. VWR has the right to charge interest on all overdue sums at the rate of five percent per annum over the base lending rate of the Bank of England calculated at the date of actual payment.

  • Customers who exceed their credit limits will be asked to pay in advance for additional products until the account is settled. 
  • Delivered products will remain the property of VWR until paid for.
  • VWR has the right to deliver an order in instalments, and the customer is required to pay the invoice for each instalment within 30 days from date of invoice. 
  • Any claims for shortages must be made within 7 days of a delivery.  
  • Any claims for non-ordered items or non delivery must be made within 7 days of receiving the invoice. 

The products we supply are intended for use in accordance with the documentation and manufacturer specifications under the user’s sole responsibility. 

Products supplied by VWR cannot be used as active pharmaceutical ingredients (APIs) in human or veterinary medicine because, as a distributor, VWR does not fulfil the requirements laid down in the guidelines for GMP medicinal products for human and veterinary use.

Customers using  products from VWR  in a pharmaceutical, cosmetic, food, pesticide or any other regulated application must carry out their own internal checks and controls to ensure that the items are of an appropriate quality/ grade and in compliance with their local laws and regulations (e.g. European Pharmacopoeia) etc.   Please contact VWR if you have any questions about your intended use and if the product you wish to purchase is suitable for that purpose. In no event shall VWR INTERNATIONAL be liable if a customer uses a product that does not fit with the intended application. 

It is the customer's responsibility to comply with all applicable health, safety and other regulations and to take appropriate steps in relation to the storage, handling, sale and use of the products.

Return of Damaged or Unsuitable Goods

No goods may be returned to VWR without the authorisation of VWR.

Authorisation to return products damaged during delivery must be requested within 3 days of delivery. VWR has the right to repair and return damaged products.

Authorisation for the return of products which fail to meet current published manufacturers specifications must be requested in writing within 28 days of delivery. VWR will assist customers, at customers’ expense, to obtain any manufacturer’s warranty consistent with that granted to VWR.

Authorisation for the return of products, other than those damaged during delivery, delivered in error or those that do not meet specification, must be requested within 10 days of delivery. Credit (less a handling charge of 15% of the invoice value of all products returned subject to a minimum charge of £30) will be given for those products authorised for return which are unused and in re-saleable condition other than those in the categories shown:

  • open chemicals or diagnostics
  • refrigerated or other perishables
  • items with an expired shelf life or an expiration date too short for resale
  • any article that has been delivered direct by a third party supplier
  • discontinued items
  • items not purchased from VWR.

Authorisation will be subject to the condition that the products are returned to VWR Customer Service Centre or to the manufacturer or other source notified by VWR, by registered post if permitted, (for which VWR will supply a label) or via the VWR scheduled van service, for which a handling charge will be made for each line.

Articles that have been delivered on our behalf by a third party supplier will not be accepted back at the VWR Customer Service Centre.

Health, Safety and Liability

Risk in products will pass at the time of delivery to the customer.

The customer is responsible for unloading and transporting large and/or heavy items from delivery vans.

In view of the hazards of certain chemicals and apparatus the customer must ensure that the products purchased are in a safe condition and that a safe system of work is in place taking into account all available information. None of the products sold are intended for human consumption unless otherwise clearly stated.

In view of the wide range of uses of chemicals and apparatus, the customer will be solely responsible for determining the suitability and specification of products, services, information and advice for its purposes. VWR has the right, without notice, to supply product of different specifications, sources, and pack size to that published or ordered. The customer is required to ensure that that the use of any products supplied by VWR do not infringe third party intellectual property rights.

In view of the above, liability of VWR for any loss or damage suffered by a customer and arising by reason of defects in the products or otherwise howsoever is limited to the invoice price of the products in respect of or in relation to which loss or damage is claimed. Any liability accepted by VWR under these terms and conditions is in lieu of any warranty or condition implied by law as to the quality or fitness for any particular purpose of the products and save as provided in these terms and conditions VWR will not be under any liability, whether in contract, tort, breach of statutory duty or otherwise in respect of defects in products delivered or for any injury (other than death or personal injury caused by VWR negligence as defined in the Unfair Contract Terms Act 1977) damage or loss resulting from such defects or from any information, service or advice provided by VWR or its employees, contractors or agents. In no event shall VWR be liable for any consequential loss whether or not resulting from force majeure. The customer indemnifies VWR against any claims from its employees, contractors or agents.

Print These Terms