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NZYM broth powder, for biotechnology

Supplier: VWR Chemicals
NZYM broths are enriched media used for the growth of lambda phages.

  • Save time by using pre-mixed, ready to use media
  • Homogenous, high quality powders

NZYM broths contain NZ-amine A, a source of high quality amino acids derived from the enzymatic digestion of casein. Yeast extract in media provide vitamins and free amino acids. This formulation contains sodium chloride and magnesium sulphate that provide ions used in osmotic regulation and enzymatic reactions, including DNA replication. The rich assortment of nutrients in NZYM broth powders allows E.coli to grow rapidly because they do not need to synthesise nucleotide precursors and other factors needed for growth.

Suspend 22 g of NZYM broth powder in 1 l of purified water. Allow the powder to dissolve with gentle stirring and heating, if necessary. Adjust the pH to 7,5 with sodium hydroxide. Autoclave for 15 minutes at 15 psi and 121 °C.

Note: Alternatively, medium may be sterile-filtered. Allow the solution to cool to 50 °C or below before adding supplemental components and antibiotics.

1 l of prepared media contains 10 g of NA-amine A, 5 g of yeast extract, 5 g of sodium chloride, 2 g of magnesium sulphate.

Store NZYM broth powder at room temperature (18 to 26 °C). Store prepared medium cold (2 to 8 °C).



 

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Description Pk Pack type VWR Catalog Number Quantity
NZYM broth powder 500 g Plastic bottle for solids J866-500G
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