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HPLC system, Chromaster

Supplier: Hitachi

The VWR Hitachi Chromaster delivers highly reliable results. This is achieved with the high precision delivered by the pump, the low carry-over and high precision of the autosampler, the stability of the column oven and the sensitivity of the detectors. VWR's relationship with Hitachi started over 30 years ago as a Merck Hitachi cooperation. During this time, the robustness and reliability of the Hitachi HPLC instruments has helped to build and develop a strong partnership.
Chromaster key parameters:

  • 600 bar (60 MPa) maximum operating pressure as standard
  • Very low carry-over autosamplers. Loop injection and direct injection available with peltier thermostatted sample racks
  • Highly sensitive fluorescence detector (optimised 30 nm slit)
  • Very low drift diode array detector (1,0 × 10-4 AU hr-1)
  • Excellent gradient reproducibility
  • Automatic wavelength using mercury lamp for UV, DAD and Fluorescence Detectors
  • Unique touch screen user interface
  • Drivers available for OpenLab® CDS, Chromeleon® 6.8 and 7.X, Empower2® and Empower3® and Clarity
All preconfigured systems (with or without detector) are delivered with all necessary cables and an interface board.

Chromaster HPLC 5160 pump
Pumping system: Dual plunger reciprocating pump system; Series connection, pulsation elimination system
Operating flow rate range: 0,001 to 5,000 ml/min
Maximum operating pressures: 60 MPa (0,001 to 2,500 ml /min); 30 MPa (2,501 to 5,000 ml /min)

Chromaster HPLC 5260 autosampler
Sample capacity: 195×1 ml (optional); 120×1,5 ml (standard); 72×4 ml (optional); 2×MTP (in 96 well or 384 well) (optional)
Sample injection system: Loop injection method; (Cut injection, all volume injection, full loop injection method)
Syringe volume: 175 µl (standard)

Chromaster HPLC 5310 column oven
Temperature control system: Heating/cooling block with air circulation system
Temperature setting range: 1 to 85 °C (1 °C step)
Temperature control range: RT -15 to RT+60 °C and range of the temperature setting

Chromaster HPLC 5410 UV detector
Optical system: Double beam ratio photometric system
Light source: D₂ lamp, Hg lamp for checking wavelength
Wavelength range: 190 - 600 nm

Chromaster HPLC 5420 UV-VIS detector
Optical system: Double beam ratio photometric system
Light source: D₂ lamp, W lamp, Hg lamp for checking wavelength
Wavelength range: 190 - 900 nm

Chromaster HPLC 5430 diode array detector
Detection type: 1,024 bit PDA
Light source: D₂ lamp, W lamp, Hg lamp for checking wavelength
Wavelength range: 190 - 900 nm

Chromaster HPLC 5440 fluorescence detector
Light source: Xe lamp, Hg lamp for checking wavelength
Wavelength range: Ex: 200 to 850 nm; Em: 250 to 900 nm (change photomultiplier at 731 nm or more)
Wavelength accuracy: ±3 nm

Chromaster HPLC 5450 RI detector
Refractive index range: 1 - 1,75
Noise: ≤2,5 x 10-9 RIU
Drift: ≤0,2 x 10-6 RIU/h

Chromaster HPLC System Manager Software
For control of: VWR Hitachi Chromaster system
PC requirements: Windows 7 Professional English 32 bit version
Functions: Method creation, data acquisition, chromatograph data processing, DAD data processing and report generation




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Preconfigured complete systems
Description Pk VWR Catalog Number Quantity
Chromaster HPLC complete 600 bar system with 5160 manual purge quaternary gradient pump, 5260 standard loop autosampler, 5310 column oven and 5410 variable wavelength UV detector 1 903-0337
Chromaster HPLC complete 600 bar system with 5160 auto purge quaternary gradient pump, 5260 standard loop autosampler, 5310 column oven and 5410 variable wavelength UV detector 1 903-0396
Chromaster HPLC complete 600 bar system with 5160 manual purge quaternary gradient pump, 5280 direct injection autosampler, 5310 column oven and 5410 variable wavelength UV detector 1 903-0352
Chromaster HPLC complete 600 bar system with 5160 auto purge quaternary gradient pump, 5260 standard loop autosampler, 5310 column oven and 5410 variable wavelength UV detector 1 903-0354
Chromaster HPLC complete 600 bar system for narrow bore columns with 5160 auto purge quaternary gradient pump, 5260 thermostat standard loop autosampler, 5310 column oven, 5430 diode array detector with low dispersion kit 1 903-0336
Chromaster HPLC complete 600 bar system for standard bore columns with 5160 manual purge quaternary gradient pump, 5260 thermostat standard loop autosampler, 5310 column oven, 5430 diode array detector 1 903-0593
Chromaster HPLC complete 600 bar system for standard bore columns with 5160 auto purge quaternary gradient pump, 5280 thermostat direct injection autosampler, 5310 column oven, 5430 diode array detector 1 903-0353
Chromaster HPLC complete 600 bar system for standard bore columns with 5160 manual purge quaternary gradient pump, 5260 thermostat standard loop autosampler, 5310 column oven, 5430 diode array detector 1 903-0355

Preconfigured systems without detector
Description Pk VWR Catalog Number Quantity
Chromaster HPLC base 600 bar system with 5160 auto purge quaternary gradient pump, 5260 thermostat standard loop autosampler, 5310 column oven 1 903-0340
Chromaster HPLC base 600 bar system with 5160 auto purge quaternary gradient pump, 5260 standard loop autosampler, 5310 column oven 1 903-0341
Chromaster HPLC base 600 bar system with 5160 manual purge quaternary gradient pump, 5260 thermostat standard loop autosampler, 5310 column oven 1 903-0342
Chromaster HPLC base 600 bar system with 5160 manual purge quaternary gradient pump, 5260 standard loop autosampler, 5310 column oven 1 903-0343
Chromaster HPLC base 600 bar system with 5160 auto purge quaternary gradient pump, 5280 thermostat direct injection autosampler, 5310 column oven 1 903-0356
Chromaster HPLC base 600 bar system with 5160 auto purge quaternary gradient pump, 5280 direct injection autosampler, 5310 column oven 1 903-0357
Chromaster HPLC base 600 bar system with 5160 manual purge quaternary gradient pump, 5280 thermostat direct injection autosampler, 5310 column oven 1 903-0358
Chromaster HPLC base 600 bar system with 5160 manual purge quaternary gradient pump, 5280 direct injection autosampler, 5310 column oven 1 903-0359
Chromaster HPLC GPC base system for gel permeation chromatography with 5160 auto purge quaternary gradient pump, 5260 thermostat loop autosampler, 5310 column oven. THF resistant parts included 1 903-0338
Chromaster HPLC normal phase base system with 5160 auto purge quaternary gradient pump, 5260 standard loop autosampler, 5310 column oven. Hexane/Heptane resistant parts included 1 903-0339

Main modules
Description Pk VWR Catalog Number Quantity
Chromaster HPLC 5410 UV variable wavelength detector 1 903-0524
Chromaster HPLC 5420 UV/VIS variable wavelength detector 1 903-0525
Chromaster HPLC 5430 diode array detector without flow cell 1 903-0597
Chromaster HPLC 5440 fluorescence detector 1 903-0527
Chromaster HPLC 5440 standalone flourescence detector including UI pad, analogue output, detector signal cable and power unit 1 903-0360
Chromaster HPLC 5450 refractive index detector 1 903-0528
Chromaster 5610 mass selective detector, including control software (MSD System Manager, English Version) 1 903-0397
ELSD 90 HPLC low temperature evaporative light scattering detector, 230 V, EU-plug 1 903-0271
Chromaster HPLC 5110 pump with manual purge valve. 400 bar max. operating pressure (9,99 ml/min max. flow rate) 1 903-0500
Chromaster HPLC 5110 pump with auto-purge valve. 400 bar max. operating pressure (9,99 ml/min max. flow rate) 1 903-0501
Chromaster HPLC 5160 pump with manual purge valve. 600 bar max. operating pressure (4,99 ml/min max. flow rate) 1 903-0554
Chromaster HPLC 5160 pump with auto-purge valve. 600 bar max. operating pressure (4,99 ml/min max. flow rate) 1 903-0555
Chromaster HPLC 5260 autosampler with 175 µl syringe. 600 bar max. operating pressure 1 903-0556
Chromaster HPLC 5260 autosampler with thermostat and 175 µl syringe. 600 bar max. operating pressure 1 903-0557
Chromaster HPLC 5280 autosampler with peltier cooling unit and 100 µl syringe. 600 bar max. operating pressure 1 903-0345
Chromaster HPLC 5280 autosampler with 100 µl syringe. 600 bar max. operating pressure 1 903-0344
Chromaster HPLC 5310 column oven for max. 3 x 300 mm columns 1 903-0520
Chromaster HPLC 6310 column oven for max. 6 x 150 mm columns or max. 3 x 300 mm columns 1 903-0361
Chromaster organiser for solvent placement and power supply 1 903-0537
Chromaster system manager software for method creation, data acquisition, chromatograph data processing, DAD data processing and report generation 1 906-0144

System accessories
Description Pk VWR Catalog Number Quantity
Chromaster HPLC low pressure quaternary gradient unit. Includes proportioning valves, conventional mixer and capillaries. For Chromaster HPLC 5110 and 5160 pumps 1 903-0562
Chromaster HPLC built-in dedicated mini pump for washing the plunger of the Chromaster HPLC 5110 and 5160 pumps 1 903-0563
Chromaster HPLC 6-channel hexane resistant degassing unit with 480 µl degassing chambers for use with both Chromaster HPLC 5110 and 5160 pumps 1 903-0567
Chromaster HPLC hexane resistant check valve for Chromaster HPLC 5110 and 5160 pumps 1 903-0559
Chromaster HPLC Interface Control Board for Chromaster system control. USB cable and e-line cable (0,5 m) included. Built into the autosampler upon installation in laboratory. If no autosampler is used, then the interface box (S) must be ordered instead 1 903-0545
Chromaster HPLC graphical user interface (GUI) touch screen controller for Chromaster 5000 series for integrated module control 1 903-0546

HPLC control interfaces for Chromaster
Description Pk VWR Catalog Number Quantity
Chromaster system manager software for method creation, data acquisition, chromatograph data processing, DAD data processing and report generation 1 906-0144
Chromaster HPLC Interface Control Board for Chromaster system control. USB cable and e-line cable (0,5 m) included. Built into the autosampler upon installation in laboratory. If no autosampler is used, then the interface box (S) must be ordered instead 1 903-0545
Chromaster HPLC graphical user interface (GUI) touch screen controller for Chromaster 5000 series for integrated module control 1 903-0546
OpenLAB CDS EZChrom edition including 1 year Software Maintenance Agreement + Hitachi LC Control 1 SET 906-0145
OpenLAB CDS EZChrom edition including 1 year Software Maintenance Agreement + Hitachi LC Control + Hitachi DAD 1 SET 906-0146

PC for software installation
Description Pk VWR Catalog Number Quantity
Dell™ OptiPlex™ 7040 Minitower 64Bit/8Gb with Intel™ i5 Quad Core™ and Intel® Q170 Chipset. Includes Dell™ mouse and international keyboard. 12 MUI languages preloaded. Three years Dell™ Warranty Next Business Day On Site plus two year LabService guarantee. Dell™ monitor E2014H 20" wide is included 1 906-0210

Fraction collector
Description Pk VWR Catalog Number Quantity
Foxy R1, stand-alone package, includes diverter valve and rack for 18 mm Ø ext. tubes, interface and remote cable, E-DIO cable, flow rates up to 25 ml/min (with 1/16" outlet tubes) 1 905-0533

Control licences for Chromaster with existing software set-up
Description Pk VWR Catalog Number Quantity
Chromaster driver control licence for Waters Empower2® or Empower3® 1 906-0156
Chromaster control licence for Chromeleon™ 6.8 1 906-0185
Chromaster control licence for Chromeleon™ 7.X 1 906-0186
OpenLab CDS EZChrom edition licence for additional Hitachi HPLC on the same PC 1 SET 906-0147
OpenLab CDS EZChrom edition licence for additional Hitachi HPLC with Diode Array on the same PC 1 SET 906-0148
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SPECIFICATIONS

Model Foxy R1
External outputs Pump pause/stop and event (tube change)
Max. flow rate 25 ml/min
Switch valves Diverter valve (3-way)
W×D×H (mm) 311×330×355
Weight (kg) 7,1

ACCESSORIES

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Accessories
Description Pk VWR Catalog Number Quantity
Chromaster HPLC 6-channel hexane resistant degassing unit with 480 µl degassing chambers for use with both Chromaster HPLC 5110 and 5160 pumps 1 903-0567
Chromaster HPLC valve mounting kit 1 903-0579
Rheodyne manual injection valve 600 bar 7725I including start cable 1 RHEO7725I-188
Chromaster HPLC 3-column selection Rheodyne® valve for Chromaster HPLC 5310 oven. Pressure stability to 345 bar. For 1/16" capillaries. Power unit, control board and tubes are included. One valve per oven only 1 903-0521
Chromaster HPLC 2-position, 6-port Rheodyne® valve for Chromaster HPLC 5310 oven. Pressure stability to 345 bar. For 1/16" capillaries. Power unit, control board and tubes are included 1 903-0522
Chromaster HPLC column management system for tracking column usage and recording many parameters. Three column electronic data tags are included 1 903-0523
Chromaster HPLC 6 column selector Rheodyne® valve for Chromaster HPLC 6310 oven. 6 position/14 ports valve with 1/32" ports. Control PCB, all capillaries (0,1 mm ID) and fittings are included to connect to 1/16" column, pre-heater and flow cell 1 903-0351
Chromaster HPLC adaption kit for THF for 5160 and 5110 Chromaster pumps with manual purge valve. (max pressure usage 400 bar) 1 903-0568
Chromaster HPLC adaption kit for THF for 5160 and 5110 Chromaster pumps with auto purge valve. (max pressure usage 400 bar) 1 903-0569
Chromaster HPLC THF resistant vespel packing for Chromaster conventional mixer. (max pressure usage 400 bar) 1 903-0570
Chromaster HPLC sample rack for 120×1,5 ml sample vials. Supplied as standard with the Chromaster 5210 and 5260 autosamplers 1 903-0511
Chromaster HPLC sample rack for 72×4 ml sample vials for the Chromaster 5210 and 5260 autosamplers 1 903-0512
Chromaster HPLC sample rack for 195×1 ml sample vials. Rack for ambient use for the Chromaster 5210 and 5260 autosamplers 1 903-0513
Chromaster HPLC sample rack for 2×96-well or 384-well micro titre plates for the Chromaster 5210 and 5260 autosamplers 1 903-0514
Chromaster HPLC sample rack for 120×1,5 ml sample vials. Supplied as standard with the 5210 and the 5260 thermostat autosamplers 1 903-0406
Chromaster HPLC sample rack for 72×4 ml sample vials for the Chromaster 5210 and 5260 autosamplers 1 903-0516
Chromaster HPLC sample rack for 195×1 ml sample vials for the Chromaster 5210 and 5260 autosamplers 1 903-0517
Chromaster HPLC sample rack for 2×96-well or 384-well microtitre plates for the Chromaster 5210 and 5260 autosamplers 1 903-0518
Chromaster HPLC 70 µl syringe kit for Chromaster HPLC 5260 and 5210 autosamplers 1 560-0173
Chromaster HPLC 175 µl syringe kit for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0415
Chromaster HPLC 700 µl syringe kit for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0560
Chromaster HPLC 3000 µl syringe kit for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0596
Chromaster HPLC hexane resistant autosampler washing pump for Chromaster HPLC 5260 autosamplers 1 903-0571
Chromaster HPLC sample loop kit (5 µl) for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0573
Chromaster HPLC sample loop kit (10 µl) for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0574
Chromaster HPLC sample loop kit (20 µl) for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0575
Chromaster HPLC sample loop kit (100 µl) for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0576
Chromaster HPLC sample loop (200 µl) for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0577
Chromaster HPLC sample loop (1000 µl) for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0561
Chromaster HPLC sample loop (4000 µl) for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0578
Chromaster HPLC 2-channel degassing unit. Only needed if pump degasser is not used for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0519
Chromaster HPLC THF resistant autosampler kit for Chromaster HPLC 5260 and 5210 autosamplers 1 903-0572
Chromaster HPLC thermo cell. Flow cell control unit for 5410 UV detector and 5420 UV-VIS detector is also required 1 903-0529
Chromaster HPLC thermostat cell control unit. Necessary for thermo cell for 5410 UV detectors and 5420 UV-Vis detectors 1 903-0535
Chromaster HPLC high pressure semi-micro flow cell, 150 bar, 3,2 µl, 5 mm for 5410 UV detectors and 5420 UV-Vis detectors 1 903-0581
Chromaster HPLC preparative flow cell, 5 mm, 2,6 µl, 10 bar for 5410 UV detectors and 5420 UV-Vis detectors 1 903-0583
Chromaster HPLC low dispersion kit with semi-micro flow cell, 150 bar, 3,2 µl, 5 mm for 5410 UV detectors and 5420 UV-Vis detectors 1 903-0589
Chromaster HPLC standard flow cell for 5430 DAD 13 µl volume with 10 mm optical path length 1 903-0320
Chromaster HPLC thermo cell. The thermostat cell control unit for 5430 diode array detector is also required 1 903-0530
Chromaster HPLC thermostat cell control unit. Necessary for thermo cell for 5430 diode array detector 1 903-0536
Chromaster HPLC high pressure semi-micro flow cell, 150 bar, 3,2 µl, 5 mm 1 903-0584
Chromaster HPLC preparative flow cell, 5 mm, 2,6 µl, 10 bar 1 903-0586
Chromaster HPLC semi-micro flow cell, 150 bar, 3,2 µl, 5 mm kit including flexible capillary set 1 903-0590
Chromaster HPLC thermostat flow cell. Thermostat flow cell control unit for 5440 fluorescence detectors is also required 1 903-0532
Chromaster HPLC thermostat flow cell control unit. For use with thermostat flow cell for 5440 fluorescence detectors 1 903-0533
Chromaster HPLC AC adapter (150 W) 1 903-0538
Chromaster HPLC AC adapter (60 W) 1 903-0539
Chromaster HPLC user interface key pad for 5160 and 5110 Chromaster pumps. Mounting parts included 1 903-0540
Chromaster HPLC user interface key pad for 5280 and 5260 Chromaster autosamplers. Mounting parts included 1 903-0541
Chromaster HPLC user interface key pad for 6310 and 5310 Chromaster column ovens. Mounting parts included 1 903-0542
Chromaster HPLC user interface key pad for 5410 Chromaster UV detectors and 5420 Chromaster UV-VIS detectors. Mounting parts included 1 903-0543
Chromaster HPLC user interface key pad for 5440 Chromaster flourescence detectors. Mounting parts included 1 903-0544
Chromaster HPLC dynamic mixer 2000 µl for 5160 and 5110 Chromaster pumps 1 903-0564
Chromaster HPLC 200 µl semi-micro mixer for flow rates of 0,4 ml/min or less for 5160 and 5110 Chromaster pumps 1 903-0507
Chromaster HPLC 700 µl static mixer for conventional use with flow rates between 0,4 and 1,8 ml/min for 5160 and 5110 Chromaster pumps 1 903-0565
Chromaster HPLC manual injector holder panel for 5160 and 5110 Chromaster pumps. Manual injector valve must be ordered seperately 1 903-0508
Chromaster HPLC column holder to be used if oven is not included in configuration 1 903-0566
Chromaster HPLC 1-channel analogue signal output unit 1 903-0534
Chromaster HPLC interface box (S). AC adapter (60 W) is required 1 903-0548
Chromaster HPLC USB-analogue input device. Maximum 2 USB-AID allowed per system. Interface Control Board is required 1 903-0549
Chromaster HPLC 2-channel analogue signal output unit 1 903-0531
Chromaster HPLC adapation plate for placing other modules in a Chromaster instrument configuration 1 903-0588
Chromaster HPLC tubing kit for two tower configuration. Includes tubing, capillaries and cables for when two Chromaster 5440 fluorescence detectors or two Chromaster detectors are installed 1 KIT 903-0587
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