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PerfeCTa NGS Quantification Technology

PerfeCTa NGS Quantification Technology
  • Accurate and sensitive method for NGS library quantification
  • Stabilized, prediluted standards for convenient use
  • Consistency across a broad range of samples
  • Sensitivity of detection to quantify low concentration libraries
  • Quantification for either Illumina or Ion Torrent

Accurate quantification of the number of amplifiable library molecules is the most critical step in the NGS workflow in obtaining high quality read data with next-generation sequencing technologies. The PerfeCta NGS Library Quantification Kit uses real-time PCR to specifically quantify library molecules that possess the appropriate adapter tag at each end. These are the suitable template molecules for either emulsion PCR used for the Ion Torrent platform or Bridge PCR used for Illumina NGS platforms.