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Comparison of two colorimetric assays in the detection of cytotoxicity in fibroblasts initiated by apoptotic inducers

Comparison of two colorimetric assays in the detection of cytotoxicity in fibroblasts initiated by apoptotic inducers
G-Biosciences offers several colorimetric cell-based proliferation and toxicity assays that have been optimised for microplates.

The assays allow for many samples to be analysed rapidly and simultaneously using compounds that stain the cells directly or that are metabolized into colored products

Cytoscan SRB assay is based on the sulforhodamine B dye assay that is reported to be a standard assay of the National Cancer Institute and National Institute of Health. SRB is a bright-pink aminoxanthene dye that binds to basic amino acids of cellular proteins under mild acidic conditions, and dissociates under basic conditions. SRB binding is stoichiometric and the amount of dye extracted from stained cells is directly proportional to the total protein mass and therefore correlated to cell number1,2,3.