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Biological buffers

Biological buffers
Practical tips for preparing buffer solutions
  • Buffer characteristics
  • Useful pH range
  • Preparing buffer solutions
  • Common buffer solutions

Many biochemical processes are markedly impaired by even small changes in the concentrations of free H + ions. It is therefore usually necessary to stabilise the H + concentration in vitro by adding a suitable buffer to the medium, without, however, affecting the functioning of the system under investigation.

A buffer keeps the pH value of a solution constant by taking up protons that are released during reactions, or by releasing protons when they are consumed by reactions.

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