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Taq DNA Polymerase

Supplier: VWR Chemicals

VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' - 3' DNA polymerase and a double strand 5' - 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning. VWR® Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing. A glycerol-free Taq DNA Polymerase is also available for automation and freeze drying.

  • Most suitable choice for routine applications
  • High performance, thermostable DNA polymerase
  • Optimal for TA cloning
Taq DNA polymerase concentration: 5 Units/μl

10X Key Buffer: Tris-HCl pH 8,5, (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween-20®
10X Extra Buffer: Tris-HCl pH 8,3, KCl, 15 mM MgCl₂, 1% Triton X-100
10X Mg-Free Key Buffer: Tris-HCl pH 8,5, (NH₄)₂SO₂, 1% Tween-20®
10X Mg-Free Extra Buffer: Tris-HCl pH 8,3, KCl, 1% Triton X-100

EU = Units

Delivery information: VWR® Taq DNA Polymerase is usually supplied with either or both Key Buffer and Extra Buffer. Key Buffer (NH4+) gives a superior amplification signal (high yield) and minimises the need for optimisation of the Mg2+ concentration or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K+) buffer. Extra Buffer promotes high specificity but careful optimisation of primer annealing temperatures and Mg2+ concentrations may be required.




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Description Pk VWR Catalog Number Quantity
Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 250 EU 733-1300
Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 500 EU 733-1301
Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 1.000 EU 733-1302
Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 2.500 EU 733-1819
Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 5.000 EU 733-1820
Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 10.000 EU 733-1303
Taq DNA Polymerase, 10X MgCl₂-free Key Buffer, 25 mM MgCl₂ 500 EU 733-1311
Taq DNA Polymerase, 10X MgCl₂-free Key Buffer, 25 mM MgCl₂ 1.000 EU 733-1312
Taq DNA Polymerase, 10X MgCl₂-free Key Buffer, 25 mM MgCl₂ 2.500 EU 733-1313
Taq DNA Polymerase, 10X MgCl₂-free Key Buffer, 25 mM MgCl₂ 10.000 EU 733-2009
Taq DNA Polymerase, 10X MgCl₂-free Extra Buffer, 25 mM MgCl₂ 500 EU 733-1304
Taq DNA Polymerase, 10X MgCl₂-free Extra Buffer, 25 mM MgCl₂ 1.000 EU 733-1305
Taq DNA Polymerase, 10X Tween-free Key Buffer (15 mM MgCl₂), 10X Triton-free Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 500 EU 733-2407
Taq DNA Polymerase, 10X Tween-free Key Buffer (15 mM MgCl₂), 10X Triton-free Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 1.000 EU 733-1307
Taq DNA Polymerase, 10X Tween-free Key Buffer (15 mM MgCl₂), 10X Triton-free Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 10.000 EU 733-1823
Red Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 500 EU 733-2408
Red Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 1.000 EU 733-2409
Red Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 2.500 EU 733-1323
Red Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 10.000 EU 733-1834
Taq DNA Polymerase, glycerol-free, without Key or Extra Buffer, 25 mM MgCl₂, 200 000 units 1 KIT 733-2038
Taq DNA Polymerase, glycerol-free, without Key or Extra Buffer, 25 mM MgCl₂ 5.000 EU 733-1999
Taq DNA Polymerase, glycerol-free, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 500 EU 733-2410
Taq DNA Polymerase, glycerol-free, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 1.000 EU 733-1817
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