ACE® Knowledge Notes

How to Determine Column Volume and Porosity

This Knowledge Note explains how to calculate and experimentally determine column volume and porosity.

How to Determine Extra Column Dispersion and Extra Column Volume

Extra column volume and dispersion can seriously affect column performance. Here we explain why, how to experimentally measure and approaches to help reduce their impact.

Gradient Method Translation Using the ACE LC Translator

The free to download ACE LC Translator can be used to simplify translating gradient methods between different format columns.

How to determine dwell volume of an HPLC or UHPLC system

Determining the dwell volume of an LC system is very useful as it is the basis of gradient elution method transfer.

Understanding the Relationship Between Particle Size, Performance and Pressure

Have you ever been confused as to the best solution to a certain separation problem? We explain the effect of these parameters on performance.

The Power of Stationary Phase Selectivity

One of the key variables in you method development is stationary phase selectivity. You can learn more about this important tool.

A Simple Step-by-Step Protocol for HILIC Method Development

If you are looking for a clear introduction to the practicalities of HILIC, this is a comprehensive guide for those unfamiliar with this mode of HPLC.

Creating the Correct Environment for Analysing Reducing Sugars

How you can create the correct environment for the analysis of monosaccarides and reducing disaccharides

The Importance of Sample Diluent in HILIC

Your choice of sample diluent in HILIC can have a profound effect on the chromatography.  This note explains why.

Column Equilibration in HILIC Mode

It is essential to fully equilibrate your HILIC columns prior to analysis to ensure a stable adsorbed water layer

Practical Ultra High Performance Liquid Chromatography (UHPLC)

Learn how UHPLC can be used to dramatically increase sample throughput and produce ultra-high resolution separations of complex samples.

Gradient Chromatography and k*

This Knowledge Note introduces the key concepts of gradient chromatography and explains how to determine appropriate gradient conditions for a separation.